Recombinant Strep-Tactin with Cysteine (r-STc)
- SKU:
- GEN-NRPA17S
- Formulation:
- Lyophilized from 5mM PB (2.5mM Na2HPO4, 2.5mM NaH2PO4, pH 6.8)
- Mol. Weight:
- 13.4 kDa subunit; Tetramer 53.6 kDa
- Theory pI:
- 8.12
- Theoretical activity:
- 18.2 U / mg protein (r-STc: Biotin = 1: 4 (mol: mol))
- Activity:
- ≥ 15 U / mg protein （Improved green method）
- Purity:
- ≥95% (SDS-PAGE）
- Concentration determination:
- absorption method at 280nm, the molar absorption coefficient is 1.68 × 105 (mol / L) -1cm-1, c (g / L) = A280nm / 3.13
- Endotoxin:
- ≤5EU / mg
- Storage condition:
- -20 °C
- Storage period:
- 3 years
Description
Streptavidin is a streptavidin mutant, which can bind specifically to streptavidin. The Strep II tag is a small 8 amino acid tag (wshpqfek). Since the tag is small, it is only about 1 kDa, which generally does not affect the structure and function of the fusion protein. It is commonly used in the detection and purification of fusion protein. Compared to streptactin, streptactin has a 10 times stronger affinity for streptavidin. It can bind and dissociate with streptavidin under mild conditions and can play a good role in separation and purification. In order to be able to bind to the medium, a cysteine has been added to the C-terminus of the amino acid sequence, which can be covalently linked to the medium through a sulfhydryl group.
M ： protein molecular weight marker
Lane 1 ： r-STc subunit Lane 2 ： r-STc Tetramer
For research only！
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Recombinant Strep-Tactin (r-ST)
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