Histone H3 Monoclonal Antibody | ABM40038

Product Overwiew:

Mouse Anti-Histone H3 Monoclonal Antibody

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H3A is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from HIST1H3A lack polyA tails; instead, they contain a palindromic termination element. HIST1H3A is found in the large histone gene cluster on chromosome 6p22-p21.3.

The antibody detects endogenous Histone H3 protein.

Host:

Mouse

Sequence:

N/A

Reactivity:

Human, Mouse, Rat, Yeast

Application:

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000), IF (1:100-1:500), IP (1:200).

Used for:

WB, IHC-P, IF, IP

Kit components:

N/A

Features & Benefits:

N/A

Usage Notes:

N/A

Storage buffer:

PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

Storage instructions:

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.