MONMOUTH JUNCTION, NJ, July 26, 2021 -- Biotech Support Group reports on a recent journal article highlighting the simplicity and efficiency of their Albumin depletion sample preparation technology for BSA containing medium used in cell culture for LC-MS proteomic analysis. The citation is:
Jenull, Sabrina, et al. "The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence." Cell Reports 36.3 (2021): 109406.https://www.sciencedirect.com/science/article/pii/S2211124721008196#mmc1.
The authors report a pivotal role for the HIR histone chaperone complex in modulating virulence of the human fungal pathogen Candida albicans. Genetic ablation of HIR function alters chromatin accessibility linked to aberrant transcriptional responses to protein as nitrogen source. This accelerates metabolic adaptation and increases the release of extracellular proteases, which enables scavenging of alternative nitrogen sources. The article states for “Mass Spectrometry analysis of culture supernatants - Cell-free supernatants from 16 hours YNB-BSA (0.025% BSA) cultures grown at 30°C were used for Mass-Spec analysis. Collected supernatants were lyophilized and dissolved in 400 μl of water for AlbuVoid™ treatment for albumin depletion…” This work provides mechanistic insights into chromatin-coupled regulatory mechanisms that fine-tune pathogen gene expression and virulence. Furthermore, the data point toward the requirement of refined screening approaches to exploit chromatin modifications as antifungal strategies.
“I are very pleased to see that AlbuVoid™ was referenced for the proteomic analysis of cell culture secrotomes. As we do not use immunoaffinity, separations are efficient across all species including the depletion of BSA as was the case here” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
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About Biotech Support Group LLC
Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients.