null

LaunchWorks One-Step RT-qPCR Master Mix

Launchworks (blue) vs. Thermo Fisher (grey).

Detection of 1 copy of SARS CoV-2 E-gene target

LaunchWorks One-Step RT-qPCR Master Mix:
Sensitive, Reproducible Detection

The LaunchWorks One-Step RT-qPCR Master Mix was designed for sensitive and reproducible detection of RNA and/or DNA targets in a single multiplex RT-qPCR reaction. The 2X format was developed to guarantee simple reaction setup and robust performance. The Master Mix has been formulated and is manufactured in LaunchWorks’ ISO 13485, cGMP compliant facility, ensuring stringent production and quality control measures.

Features

• Ability to run multiplexed reactions
• Robust sensitivity for high and low-copy targets
• ISO 13485:2016 certified facility

• Built in tolerance to common clinical sample inhibitors
• Compatible with a range of RT-qPCR equipment
• Manufactured under cGMP conditions

 

Only three steps from sample prep (i.e. RNA extraction) to getting results from your RT-qPCR results:

1) reagent mixing 2) plate dispensing 3) RT-qPCR method running

Broad Dynamic Range

A master mix must be reliable across a dynamic range of copy numbers for any desired target. This is especially true in a clinical setting where pathogens may be present at low titers. Every lot of this 2X One-Step RT-qPCR Master Mix undergoes quality control performance testing across 6 magnitudes of SARS CoV-2 E-gene template RNA. The master mix is designed to use only a single assay protocol to test for either (or both) RNA and DNA and requires no passive dye.

Figure: Sensitive and reproducible detection across 6 magnitudes of SARS CoV-2 E-gene RNA template, testing down to 27 copies. Amplification plot using the 2X One-Step RT-PCR Master Mix, and the 2019-nCoV Primer Probe Panel (Charité/Berlin) available from IDT, with FAM probe detection.

High Sensitivity

Performance of LaunchWorks produced master mix is comparable to ThermoFisher’s 4X TaqPath 1-Step Multiplex Master Mix. As the copy number of E-gene present in the assay decreases, the LaunchWorks master mix amplification curve plateau reads stronger than the Thermo Fisher master mix.

Figure: Detection of 1 copy of SARS CoV-2 E-gene target in extracted reference material - Launchworks 2X One-Step RT-PCR Master Mix (blue) vs. Thermo Fisher 4X TaqPath 1-Step Multiplex Master Mix (grey).

Consistent Performance

Strict lot-to-lot quality control for critical components ensures consistent performance of the LaunchWorks 2X One-Step RTqPCR Master Mix.