DirectPCR® DNA Extraction System

Full line of single-tube systems for rapid, convenient, and reliable preparation of DNA from mouse tails, ears, yolk sacs, and culture cells. 

DirectPCR® Lysis Reagent (Mouse Tail)

DirectPCR Lysis Reagent (Cell)

DirectPCR Lysis Reagent (Ear)

DirectPCR Lysis Reagent (Yolk Sac)

The innovative system developed by Viagen Biotech allows the resulting DNA crude extracts to be ready for genomic PCR for genotyping in less time and less hands-on involvement.
Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. Our lysis reagents completely eliminate any solution transfer or tube-opening steps, providing you with substantial extra time and less risk. Our best-selling product, the 102-T, allows for up to 500 reactions at a price of just 30 cents per tail.

Genotyping without DNA isolation

Brief procedure:

1.  Lyse tails in DirectPCR® Lysis Reagent.
2.  Incubate for 45 min at 85°C.
3.  PCR genotyping with 1 µl lysates.

Detailed protocols:  Tail, Ear, Yolk Sac, and Cultured cells.

The DirectPCR® system offers advantages and savings over conventional protocols that include:
 - Time: Virtually no hands-on time. Crude tail lysates for PCR. 
 - Cost: Perform reactions at just 30 cents per tail.  
 - Safety: No organic reagents.
 - Environmental: Less waste (organic reagents, tubes, tips, etc...)
 - Reliability: Virtually 100% success rate with high yields.