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Tools to Study the Role of Bacterial Agents in Rheumatoid Arthritis

1. Collagen Antibody-Induced Arthritis (CAIA)

CAIA is the fastest, most consistent arthritis model to study the pathogenic roles of bacterial agents involved in RA and other autoimmune diseases. Arthritis is induced in mice within 24 - 48 hours by a single injection of a sub-arthritogenic dose of Arthrogen- CIA® monoclonal antibody cocktail followed by (a) IP injection or (b) oral administration of bacterial toxins such as LPS, MAM, or SEB. Interestingly, oral administration of LPS triggers arthritis in old mice (8 months old) but not in young mice (8 weeks old), indicating significant age-associated changes in the mucosal barrier function as seen in Figure (c).

a) Arthritis Triggered by IP Injection of LPS (1)

Procedure

Day 0: IV injection of Arthrogen-CIA® monoclonal antibody cocktail.

Day 5: IP injection of LPS (50 µg) or test samples.

 

NOTE: LPS can be injected up to 5 days after mAb injection. Serum cytokine level is a marker for the pathogenic effect of test samples.

b) Arthritis Triggered by Oral Administration of LPS (2)

Procedure
Day 0: IV injection of Arthrogen-CIA® monoclonal antibody cocktail.

Day 1: Oral administration of LPS (1 - 3 mg/mouse) with and without an LPS-adsorbent.

 

NOTE: Severity of arthritis (day 7) correlates with serum IL-6 levels 6 hours after LPS oral administration.