Sample Preparation – Liquid Biopsy

Proteomic Sample Prep for

  • Urine
  • Blood/Serum/Plasma
  • Saliva/Sputum
  • Synovial Fluid
  • BALF
  • Exosomes

With the emergence of liquid biopsy biomarkers, BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using one of these two solutions, researchers will be able to improve efficiency and outcomes regardless of the analytical platform. This will be especially beneficial for discovery investigations requiring cost effective and efficient sample prep methods essential for expanding proteomics into routine healthcare.



Urine Protein Enrichment & Concentration

  • Linearly scaleable, unlike ultrafiltration
  • Alternative to solvent/ alcohol precipitation
  • On-bead digestion protocols
  • Applicable to
    >1 & 2 DE
  • The eluted fractions retain their enzymatic and biological activity


Selectively Voids Albumin, Binds Low Abundance Proteome

  • Albumin voids in flow through, >95%
  • Low abundance enrichment equivalent or better than hexapeptides or antibodies
  • On-bead digestion protocols, efficient LC-MS workflows
  • Disposable, cost-effective, no column regeneration or cross-contamination

Notable Reference

For serum & synovial fluid

Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum

AlbuVoid™ PLUS

Albumin and IgG Depletion From Serum/Plasma for Proteomics

  • IgG removal >90% (70-80% of total Immunoglobulins removed)
  • Albumin removal >95%
  • Seamless and simple < 1 hour protocol
  • Low abundance enrichment equivalent to immuno-affinity
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Works for most species tested including human, sheep, rat, mouse, bovine
  • On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
  • Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.


Selectively Binds Albumin

  • Removes serum albumin >90%
  • Economical small ligand surface architecture (not dye-based), bio-affinity performance
  • Species agnostic

AlbuSorb™ PLUS

Selectively Binds Albumin & Immunoglobulin

  • > 85% Albumin, 85% IgG depleted from 25 μl serum

Notable References

For Urine exosomes

Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis

For Synovial Fluid

PRELP protein inhibits the formation of the complement membrane attack complex

For Serum exosomes

Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy


Hemoglobin Depletion For Erythrocyte Proteomics

  • Hemoglobin voids in flow-through, applicable to red cells, heavily hemolyzed serum, whole blood and dried blood spot (DBS) card
  • Low abundance protein and enzyme enrichment
  • Consumable, cost-effective
  • Mild elution maintains native structure with retained enzymatic, functional and bioactivities
  • Compatible with LC-MS, activity-probe profiling and virtually all proteomic analyses
  • Species agnostic

Notable References

Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.

Annotating N termini for the Human Proteome Project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome.

HemoVoid™ Blood Card Kit

The HemoVoid™ Blood Card kit substantially reduces hemoglobin interference from dried blood spot protein analytes

NuGel™ HemogloBind™

Removes Hemoglobin Interference

  • Highly specific for hemoglobin binding
  • depletion from hemolyzed serum, dura, BALF, and whole blood
  • supports biomarker quantitative analysis

Notable References

For Bronchoalveolar Lavage Fluid (BALF)

Proteomic Studies in Acute Hypoxic Respiratory Failure.

For Dried Blood Spot (DBS)/Whole Blood

A simple assay for glutathione in whole blood

For hemolyzed serum/plasma exosomes

Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis.

For hemolyzed serum/plasma

Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis.

HemogloBind™ Blood Card Kit

Hemoglobin Depletion and Protein Enrichment From Dried Blood Spots

  • Dried blood spots are useful for low volume analyses, and simple collection and transport
  • Protocols suitable for inexpensive whole blood card systems, no need for cell separation
  • Hemoglobin binding >90%, with 30-45 minute spin-filter format
  • Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most volumes
  • Blood proteins and enzymes are enriched for biomarker and proteomic investigations.
  • Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat, mouse, etc.
  • High throughput easicly scalable.


Lipid Adsorption & Clarification

  • Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
  • Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
  • A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids

Notable References

Plasma/Serum Protein Biomarkers

For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }

Monoclonal antibody to a cancer-specific and drug-responsive hydroquinone (NADH) oxidase from the sera of cancer patients.

For saliva/sputum/tracheal swab

A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

Isolation of M. tuberculosis RNA from Sputum

NuGel™ PBA
NuGel™ PBA Kit

Glycoprotein Enrichment Using Phenyl Boronic Acid

  • Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
  • Consumable, no column regeneration
  • Species and tissue agnostic
  • Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
  • Binds biomolecules containing 1,2 cis-diol groups
  • Chemically derived, ideal for glyco-proteomic applications
  • NuGel™ polymer coating, porous silica based
  • Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)

Notable Reference

For saliva

Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva