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OVA Monoclonal Antibodies & Elisa Kits

Ovalbumin From Chick Egg White

     

Products

 

Quantity

Catalog #

 

Ovalbumin From Chick Egg White

50 mg, Lyophilized

30211

 

Low Endotoxin Ovalbumin From Chick Egg White

10 mg, Lyophilized

3022

 
         

Mouse Anti-OVA IgE and IgG Monoclonal Antibodies

   

Product

Mast Cell Activation

Hypersensitivity Reaction

Quantity

Catalog #

IgE, Clone E-C1

Yes

Yes

1 mg, Lyophilized

3006

IgE, Clone E-G5

No

No

1 mg, Lyophilized

3007

IgG1, Clone L71

No

No

1 mg, Lyophilized

3008

         

Mouse Anti-OVA Antibody and Total Immunoglobulin ELISA Kits

   

Products

Anti-HDM (Catalog #)

Total Immunoglobulin (Catalog #)

 

IgE Antibody Assay Kit

3004

3005

   

Serum IgE Antibody Assay Kit

3010

-

   

IgG Antibody Assay Kit

3011

3023

   

IgG1 Antibody Assay Kit

3013

3025

   

IgG2a Antibody Assay Kit

3015

3026

   

IgG2b Antibody Assay Kit

3016

3027

   

IgG2c Antibody Assay Kit

3029

-

   

IgG3 Antibody Assay Kit

-

3028

   

IgM Antibody Assay Kit

3017

3024

   

IgA Antibody Assay Kit

3018

3019

   

 

Typical mouse OVA asthma models take several weeks to induce asthma because anti-OVA antibody production is essential. To bypass this rate-limiting step, a newly-developed asthma mouse model uses an anti-OVA IgE monoclonal antibody (mAb) rather than OVA for sensitization, resulting in a one-week study as shown in Figure 1 (1,2). Chondrex, Inc. provides mouse anti-OVA IgE mAbs, allergenic E-C1 and non-allergenic E-G5, anti-OVA IgG mAb L71, and related kits for studying allergic diseases, such as in-vivo hypersensitivity reactions for in-vitro and in-vivo experiments.

General Protocols for Inducing Asthma in Balb/c Mice

OVA/Alum-Induced Asthma

Anti-OVA/IgE-Induced Asthma

Interestingly, anti-OVA IgG synergistically develops an allergic reaction with non-allergenic IgE. This result indicates that anti-allergen IgG may play pathogenic roles in allergic diseases by forming multivalent antigen complexes which bind to IgE on the surfaces of mast cells, rather than competing with IgE for its allergenic epitopes (3).

Footpad Swelling in Mice with Anti-OVA IgEs

Balb/c mice received 10 μg of anti-OVA monoclonal IgE antibodies (allergenic E-C1 and non-allergenic E-G5) and IgG1 (L71) by IV injection, then were challenged with OVA (50 μg) by intradermal injection at the footpad after 24 hours. Footpad thickness was determined with a Loop Handle Dial Thickness Gauge and shown in mm. Sensitization with E-C1 resulted in a hypersensitivity reaction as indicated by footpad swelling (black). In contrast, E-G5 (orange) and L71 (blue) did not induce individual hypersensitivity reactions.
However, co-injection of E-G5 and L71 synergistically induced a hypersensitivity reaction (green) commensurate to E-C1.