Mitochondrial Membrane Potential Assay Kit (JC-1) | KTA4001

Product Overwiew:

Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1) provides a simple method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential.

Background:

Mitochondria, the site of most energy production in eukaryotic cells, have a double membrane structure: an outer membrane and a folded inner membrane. Across the inner membrane of intact mitochondria there is a voltage gradient with the inside negative and the outside positive. Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis.

Used for:

N/A

Kit components:

• JC-1 Stain
• CCCP (10 mM)
• Assay Buffer (5×)

Features & Benefits:

• Measure mitochondrial membrane potential as an indicator of cell health.
• Use JC-1, a fluorescent, lipophilic, cationic dye.
• Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in poor health.
• Optimized for flow cytometery or fluorescent microscopy.
• Contain CCC (Potent mitochondrial oxidative phosphorylation uncoupler).

Usage Notes:

• JC-1 Stain is difficult to prepare due to its low solubility. Make sure JC-1 Stain is completely thawed and warmed to room temperature before using.
• JC-1 Stain is light senstive. Incubations need to be done in the dark.

Storage buffer:

N/A

Storage instructions:

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.