Human Anti-IL6 autoantibody ELISA Kit | KTE62492

Product Overwiew:

This Human Anti-IL6 autoantibody ELISA Kit employs a two-site sandwich ELISA to quantitate Anti-IL6 autoantibody.

Background:

IL-6 is a multifunctional protein produced by lymphoid and non-lymphoid cells, and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these various cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. On the basis of its various activities, IL-6 has also been called interferon-β2 (IFN-β2), 26 kDa protein, B-cell stimulatory factor-2 (BSF-2), hybridoma/plasmacytoma growth factor, hepatocyte stimulating factor, cytotoxic T-cell differentiation factor, and macrophage-granulocyte inducing factor 2A (MGI-2A).

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Anti-IL6 autoantibody ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-IL6 autoantibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-IL6 autoantibody and incubated. The competitive inhibition reaction is launched between with HRP labeled Anti-IL6 autoantibody and unlabeled Anti-IL6 autoantibody with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Anti-IL6 autoantibody in the sample. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Anti-IL6 autoantibody ELISA Kit microplate
• Human Anti-IL6 autoantibody ELISA Kit standard
• Human Anti-IL6 autoantibody ELISA Kit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Anti-IL6 autoantibody ELISA Kit has high sensitivity and excellent specificity for detection of Human Anti-IL6 autoantibody. No significant cross-reactivity or interference between Human Anti-IL6 autoantibody and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.