Human Interferon alpha antibody (IFNA-Ab) ELISA Kit | KTE62491

Product Overwiew:

This Human Interferon alpha antibody (IFNA-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate IFNA-Ab.

Background:

Streuli et al. (1980) showed that at least 3 different IFN-alpha genes are expressed in man. Furthermore, study of genomic DNA revealed the presence of at least 8 IFN genes.Nagata et al. (1980) found that the alpha-interferon genes are devoid of intervening sequences. Using radioactive probes from purified cDNA clones of interferons, Owerbach et al. (1981) located at least 8 leukocyte interferon genes and a fibroblast interferon gene on chromosome 9. Shows et al. (1982) found that the alpha- and beta-interferon genes are on 9p. The mapping to 9pter-q12 was accomplished by blot hybridization of cloned interferon cDNA to DNA from human-mouse cell hybrids with a translocation involving chromosome 9.

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Interferon alpha antibody (IFNA-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate IFNA-Ab in samples. An antibody specific for IFNA-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFNA-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNA-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNA-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Interferon alpha antibody microplate
• Human Interferon alpha antibody standard
• Human Interferon alpha antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Interferon alpha antibody (IFNA-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human IFNA-Ab. No significant cross-reactivity or interference between Human IFNA-Ab and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.