Human Interleukin 2 antibody (IL2-Ab) ELISA Kit | KTE62473

Product Overwiew:

This Human Interleukin 2 antibody (IL2-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate IL2-Ab.

Background:

Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

Host:

N/A

Sequence:

N/A

Reactivity:

Human

Application:

This Human Interleukin 2 antibody (IL2-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated IL2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled IL2-Ab and unlabeled IL2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL2-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Used for:

ELISA

Kit components:

• Human Interleukin 2 antibody microplate
• Human Interleukin 2 antibody standard
• Human Interleukin 2 antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits:

Human Interleukin 2 antibody (IL2-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human IL2-Ab. No significant cross-reactivity or interference between Human IL2-Ab and analogues was observed.

Usage Notes:

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.