ExKine™ Membrane and Cytoplasmic Protein Extraction Kit | KTP3005

Product Overwiew:

Abbkine ExKine™ Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic and membrane protein.

Background:

The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins. The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.

Used for:

N/A

Kit components:

• Cytoplasmic Extraction Buffer (CEB) (5×)
• Membrane Extraction Buffer (MEB)
• Protease Inhibitor (100×)

Features & Benefits:

• Versatile—suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
• Fast and convenient—non-denatured, active proteins are purified in less than two hours.
• Compatible—apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.

Usage Notes:

Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.

Storage buffer:

N/A

Storage instructions:

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.