Annexin V-AbFluor™ 555 Apoptosis Detection Kit | KTA0003

Product Overwiew:

Annexin V-AbFluor™ 555 Apoptosis Detection Kit allows the identification and quantification of apoptotic cells.

Background:

Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet.

Used for:

N/A

Kit components:

• Annexin V Binding Buffer (5x)
• Annexin V- AbFluor™ 555

Features & Benefits:

• Abbkine AbFluor™ 555 dye provides the same or better fluorescence and photostability as Alexa Fluor® 555, Cy™3, TRITC
• Annexin V- AbFluor™ 555: Abs/Em = 555/565 nm
• Suitable for flow cytometry or fluorescence microscopy

Usage Notes:

N/A

Storage buffer:

N/A

Storage instructions:

Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze.

Shipping:

Blue ice

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.