Description
abm | GFP-Tagged CENP-A & CSAP Stable Expressing HeLa Cell Line | T6345
These particular HeLa cells stably express the CENPA gene which encodes for a centromere protein. This protein is involved in targeting to the centromere.
High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures.
Biosafety:
II
Organism:
Human
Source Organ:
Cervix
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
24 - 34 hours
Seeding Density:
40,000 - 50,000 cells/cm2
Markers:
CENP-A, CSAP, GFP
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) Phenotypic analyses via microscopy
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C