abm | Immortalized GLUT4-Overexpressing Rat Skeletal Myoblast Cells (L6) | T0772

abm | Immortalized GLUT4-Overexpressing Rat Skeletal Myoblast Cells (L6) | T0772

Immortalization Method:

Immortalized by treatment with 3-methylcholanthrene

BioSafety Level:

II

Organism:

N/A

Species:

Rat

Source Organ:

Skeletal muscle

Organ Type:

Skeletal

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

20 - 30 hours

Seeding Density:

20,000 - 30,000 cells/cm10²; Recommended split ratio of 1:5 to 1:10

Markers:

Blasticidin-HCl resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

The differentiation media is Prigrow VIII, 2% FBS , and Antibiotic/Antimycotic (100X) . Cells will reach confluence after 2- 3 days in culture using the recommended seeding density. After cells are confluent, switch cultures from growth media to differentiation media, then culture for another 2- 3 days. Use myotubles on the 7th day after seeding, as myotubles may begin to lift off the plate if cultured for too long. Avoid culturing at low densities, as the L6 muscle myoblasts cells have the tendency to grow in tightly packed patches called islets. Change media every 2-3 days.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C