abm | Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4 | T0497

abm | Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4 | T0497

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying hTERT and CDK4 genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

Doubling time of approx. 23 hours

Seeding Density:

5,000 - 7,500 cells per cm10²

Markers:

Cytokeratin

Donor Age:

55

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow X series medium available at abm.

Quality Control:

Real Time PCR was used to quantify hTERT and BMI gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C