abm | Immortalized Human Carotid Artery Smooth Muscle Cells - SV40 | T0510

abm | Immortalized Human Carotid Artery Smooth Muscle Cells - SV40 | T0510

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Carotid Artery

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

30 - 35 hours

Seeding Density:

6,000 - 10,000 cells per cm10²

Markers:

alpha-Actin

Donor Age:

33

Donor Gender:

Male

Donor Ethnicity:

Hispanic

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C