abm | Immortalized Human Chondrocytes - hTERT | T0023

abm | Immortalized Human Chondrocytes - hTERT | T0023

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Normal human articular cartilage

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

41-51 hours

Seeding Density:

25,000-30,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, 1% L-glutamine, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C