abm | Immortalized Human Corneal Keratinocytes (IHCK) | T0579

abm | Immortalized Human Corneal Keratinocytes (IHCK) | T0579

Together with the Immortalized Human Corneal Stromal Fibroblasts (T0578) and the Immortalized Human Corneal Endothelial cells (T0577) isolated from the same donor and immortalized using the same platform, this uniform model system presents a valuable tool for studies involving cornea homeostasis and ophthalmologic diseases.

Immortalization Method:

Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

corneoscleral rim

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

24 hours

Seeding Density:

30,000 - 50,000 cells/cm10²

Markers:

CK19, CK12, CD36, Stromal Fibrillar Collagen Type 1, S100A4

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow X series medium available at abm.

Quality Control:

1) RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7; 2) Indirect immunofluorescence was performed to detect the cell specific marker expression CK12, CK19 and ABCG2; 3) FISH was used to examine the cytogenetic alterations in the immortalized cells.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C