abm | Immortalized Human Diploid Fibroblasts - hTERT | T1013

abm | Immortalized Human Diploid Fibroblasts - hTERT | T1013

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

30 - 35 hours

Seeding Density:

10,000 - 20,000 cells/cm10²

Markers:

N/A

Donor Age:

Fetal, 3 months

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C