abm | Immortalized Human Epidermal Keratinocytes | T0345

abm | Immortalized Human Epidermal Keratinocytes | T0345

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Abdominal Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

24-34 hours

Seeding Density:

10,000-15,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

39

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Keratinocyte Medium Kit available at abm.TM040.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C