abm | Immortalized Human Fetal Liver Cells (cBAL111) | T0789

abm | Immortalized Human Fetal Liver Cells (cBAL111) | T0789

Immortalization Method:

Immortalized with lentivirus containing hTERT

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Spindle-shaped

Passage Number:

N/A

Population Doubling:

12 days; have contact inhibition

Seeding Density:

300,000 cells/cm²

Markers:

Ampicillin & neomycine-resistant, expresses albumin, glutamin synthetase, cytokeratin 18, and cytokeratin 19.

Donor Age:

14 - 18 weeks

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, 1X L-glutamine , 1 µM dexamethason (Sigma), 10 µg/mL insulin, 5.5 µg/mL transferrin, 6.7 ng/mL selenium-X (ITS mix, Life Technology), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1. qRT-PCR for hTERT expression.
2. cBAL111 clone continued to proliferate whereas primary cells senescenced in culture.
3. Immunostaining conducted showed cells are positive for hepatic markers albumin, glutamin synthetase and cytokeratin 18, and positive for cholangiocyte marker cytokeratin 19.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C