abm | Immortalized Human Melanocytes-hTERT | T0462

abm | Immortalized Human Melanocytes-hTERT | T0462

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Skin, Foreskin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

20-30 hours

Seeding Density:

20,000-25,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

N/A

Donor Age:

5 year old

Donor Gender:

Male

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C