abm | Immortalized Human Neonatal Foreskin Keratinocytes (HKc16E6/E7-II) | T0726

abm | Immortalized Human Neonatal Foreskin Keratinocytes (HKc16E6/E7-II) | T0726

Immortalization Method:

Transfection with HPV16 E6 and E7

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

HPV-16 E6/E7 expression, neomycin resistance, and ampicillin resistance

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0726.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) RNaseH assays; 2) ODN uptake measurements; 3) Western blot; 4) RT-PCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C