abm | Immortalized Human Rheumatoid Arthritis Cartilage Progenitor Cells (RA-CPC) | T0742

abm | Immortalized Human Rheumatoid Arthritis Cartilage Progenitor Cells (RA-CPC) | T0742

Immortalization Method:

Immortalized with lentivirus containing hTERT, packaged using Lentifectin™ Transfection Reagent

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Cartilage

Organ Type:

Skeletal

Growth Properties:

Adherent

Morphology:

Spindle-shaped

Passage Number:

N/A

Population Doubling:

28 - 34 hours

Seeding Density:

1,000 cells/cm10²; recommended split ratio of 1:2 to 1:3

Markers:

Stem cell markers CD29, CD44, CD73, CD90, CD105 (weak expression)

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1% or 500µl gentamycin (Roth, HN09.1, 50mg/ml).
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) FACS

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C