abm | Immortalized Human Scalp Dermal Papilla Cells (KNU-201) | T0736

abm | Immortalized Human Scalp Dermal Papilla Cells (KNU-201) | T0736

Immortalization Method:

Co-transfection with vectors carrying SV40 & hTERT genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Skin

Organ Type:

Hair Follicle

Growth Properties:

Adherent

Morphology:

Mulit-polar

Passage Number:

N/A

Population Doubling:

24 - 30 hours

Seeding Density:

N/A

Markers:

Neomycin, Hygromycin, Wnt5a, ALP, HEY1, HEY2, FGF7

Donor Age:

35 years old

Donor Gender:

Male

Donor Ethnicity:

Korean

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR; 2) Immunocytochemistry

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C