abm | Immortalized Human Skin Fibroblast - HPV E6/E7 | T0904

abm | Immortalized Human Skin Fibroblast - HPV E6/E7 | T0904

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Bipolar

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Puromycin resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, and L-glutamine to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C