abm | Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10) | T0139

abm | Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10) | T0139

in vitro. The CD81 gene has associations with malignancies as it is localized within the tumor-suppressor gene region. Hence valuable for studies in regards to cancer.

Immortalization Method:

Transfection preformed with pSV3-SV40-T antigen plasmid DNA

BioSafety Level:

II

Organism:

CD81 -/- mice

Species:

Mouse

Source Organ:

Marrow from long bone

Organ Type:

Blood

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 6%, 6% Nu-Serum (Corning^™ 355100), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

RT-PCR was used to detect the presence of SV40 large T antigen and CD81

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C