abm | Immortalized Mouse Cementoblasts | T0047
- SKU:
- T0047
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Mouse Cementoblasts | T0047
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level:
II
Organism:
CD-1 Mouse
Species:
Mouse
Source Organ:
Mandibular First Molars
Organ Type:
Oral
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
Bone sialoprotein (BSP), Osteocalcin (OC), Osteopontin (OPN), Type I collagen
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, Penicillin/Streptomycin Solution .
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Expression of PTH-1 was assessed by Northern blot and gene-expression assay. Markers were analyzed by RT-PCR.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C