Description
abm | Immortalized Mouse Colonic Epithelial Cells-Conditionally (YAMC) | T0567
Immortalization Method:
Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
BioSafety Level:
II
Organism:
18-day-old H-2Kb-tsA58 transgenic mouse
Species:
Mouse
Source Organ:
Colon
Organ Type:
Digestive
Growth Properties:
Adherent
Morphology:
Epithelial
Passage Number:
N/A
Population Doubling:
22 - 32 hours
Seeding Density:
20,000 cells/cm10². Recommended split ratio of 1:3
Markers:
CK18
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, and Recombinant Murine IFNG to a final concentration of 5 ng/mL.
The expression of alkaline phosphatase and dipeptidyl peptidase IV can be upregulated by the addition of 1mM sodium butyrate, 1 µM Phorbol Myristate Acetate (PMA), or 1 µM Retinoic Acid (RA) to the culture medium for 5 days. Conversely, the addition of 5 µg/ml TGF-β or 1 µM Bt2GMP will downregulate the two enzymes.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) Immunohistochemical staining was used to detect the presence of SV40 large T antigen and keratin 18; 2) Biochemical assays (i.e. method of Nagatsu et al and method of Dahlquist) were used to measure brush border-associated enzymes alkaline phosphatase, dipeptidyl peptidase IV and sucrase.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C