abm | Immortalized Mouse Distal Tubule KDT3 Cells | T0641

abm | Immortalized Mouse Distal Tubule KDT3 Cells | T0641

2+-permeable non-selective cation channel, TRPV4, mostly in the basolateral membrane compared to the apical membrane. Studies have shown some association with extracellular Ca²⁺ levels and the uptake of aminoglycoside. This cell line may be used to study the effects and mechanism of aminoglycoside antibiotics permeation through TRPV4.

Immortalization Method:

Spontaneously immortalization

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

18 - 28 hours

Seeding Density:

25,000 - 30,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

RT-PCR was used to detect the presence of TRPV4 transcript

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C