abm | Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) | T0299

abm | Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) | T0299

Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells were isolated from the Cdc25a^fl/fl/Cre^- mice. The cells are suitable as a control for the mutant cells, in which CDC25A is not expressed, Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

C57/BL6 Cdc25a^fl/fl/Cre^- mouse

Species:

Mouse

Source Organ:

Skin

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm. , Penicillin/Streptomycin Solution to a final concentration of 1% and 5 ng/ml recombinant human FGF7 (Z100595).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Immunoblot for the presence of CDC25A was conducted.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C