abm | Immortalized Mouse Erythoid Progenitor Cell Line (CD36E) - HPV16 E6/E7 | T0136

abm | Immortalized Mouse Erythoid Progenitor Cell Line (CD36E) - HPV16 E6/E7 | T0136

Immortalization Method:

Infection with the lentiviral vector carrying HPV16 E6/E7 genes

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Blood

Organ Type:

Blood

Growth Properties:

Adherent

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IX Medium available from abm . To make the complete growth medium, add the following components to the base medium a final concentration: 100 ng/ml Recombinant Human Stem Cell Factor (Z100815), 10^-6 M Hydrocortisone (Sigma; H6909), 5 ng/ml Recombinant Human IL-3 (Z101295), 3 IU Recombinant Human Epo (Z101435), 10 mg/ml BSA (Sigma; A1933), 10 µg/ml Recombinant Human Insulin (Z101065), 200 µg/ml Human Holo Transferrin (G599), 900 ng/ ml Ferrous Sulfate (Sigma; 1270355), 90 ng/ml Ferric Nitrate (Sigma; F8508), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) HPV E6/E7 gene detected via PCR; 2) karyotyping was preformed

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C