abm | Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) | T0134

abm | Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) | T0134

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Leptin receptor-deficient mouse (db/db)

Species:

Mouse

Source Organ:

Bone Marrow

Organ Type:

Blood

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: a final concentration of fetal bovine serum to a final concentration of 10%, 10% Opti-MEM (gibco), 15 mM/L HEPES, and 100 µg/ml gentamycin or Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) qRT-PCR confirmed LepR deficiency; 2) Flow cytometry was used to analyze cell surface markers.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C