abm | Immortalized Mouse Melanocytes | T0466

abm | Immortalized Mouse Melanocytes | T0466

Immortalization Method:

Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

c-kit, Pax3, Sox10, MITF-m

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Fontana-Masson staining for melanocytic cell markers; 2) Tyrosine enzymatic assays determined presence of tyrosine; 3) Western blot analysis for SV40 large T antigen presence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C