abm | Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) | T0298

abm | Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) | T0298

Immortalized Mouse Mutant Epidermal Keratinocytes (CDC25A 8b) Cells were isolated from the Cdc25a^fl/fl/Cre⁺ mice in which the CDC25A gene is flanked with loxP inserted at exons 1-3 with the Krt14 promoter-driven Cre recombinase (Cre⁺) in which the expression of CDC25A is absent. abm also provides the Immortalized Mouse Epidermal Keratinocytes (CDC25A F7) Cells which may be used as a control cell line. The cells are suitable for studies on the CDC25A gene and its expression in cell cycle progression.

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

C57/BL6 Cdc25a^fl/fl/Cre⁺ mouse

Species:

Mouse

Source Organ:

Skin

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm. , Penicillin/Streptomycin Solution to a final concentration of 1% and 5 ng/ml recombinant human FGF7 (Z100595).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunoblot for the presence of CDC25A was conducted.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C