abm | Immortalized Mouse Olfactory Placode Cells (OP27) | T0787

abm | Immortalized Mouse Olfactory Placode Cells (OP27) | T0787

Immortalization Method:

Immortalized via retroviral transduction of the U19-tsA58 TAg simian virus 40 large tumor antigen

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Embryo

Organ Type:

Embryo

Growth Properties:

Adherent

Morphology:

Small clusters of cells interconnected by short processes

Passage Number:

N/A

Population Doubling:

Approximately 52 hours

Seeding Density:

N/A

Markers:

Neomycin resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Southern blot analysis; 2) RT-PCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C