abm | Immortalized Mouse Osteocytic Cells-Conditionally (IDG-SW3) | T0231

abm | Immortalized Mouse Osteocytic Cells-Conditionally (IDG-SW3) | T0231

Similar to osteocytes in vivo, these cells respond to hormonal signals such as PTH and 1,25-dihydroxyvitamin-D3. IDG-SW3 cells can be maintained in both 2D and 3D cultures and are useful for study of osteocytes at various differentiation stages. It should be noted that these cultures may contain a mixture of osteoblasts and osteocytes at different stages of differentiation and for studies requiring enriched osteocytes, the Dmp1-GFP cells should be isolated by flow cytometry (FACS).

Immortalization Method:

Isolated from long bones of transgenic mice (in which the Dmp1 promoter drives the expression of GFP).

BioSafety Level:

II

Organism:

3-month old Immortomouse+/- crossed with Dmp1-GFP+/- transgenic mouse

Species:

Mouse

Source Organ:

Long bones

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

40 - 50 hours

Seeding Density:

25,000 - 30,000 cells/cm10²

Markers:

ALP (osteoblast), E11/gp38, Dmp1 and Phex (osteocyte), SOST/sclerostin and FGF23 (late osteocyte)

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The basal medium for this cell line is Prigrow III medium available in abm. to a final concentration of 10%, 50U/ml IFN γ and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.

Quality Control:

(1) Presence and absence of SV40 large T-antigen, under permissive and non-permissive conditions, were confirmed using western blot. (2) Fluorescent imaging was used to measure Dmp1 promoter driven GFP expression in osteogenic conditions. (3) Von Kossa staining was used to show focal nodular mineralization and Alizarin red S staining was used to detect calcium deposition of the differentiated cells.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C