abm | Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) | T0504

abm | Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) | T0504

Immortalization Method:

Immortalization using polyoma middle-sized T-antigen (PmT)

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Heart

Organ Type:

Blood Vessel

Growth Properties:

Adherent

Morphology:

Elongated

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

20,000-40,000 cells/cm10²

Markers:

PECAM1, eNOS, VE-cadherin, and von Willebrand Factor

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C