abm | Immortalized Mouse Wildtype M4^+/+ Epidermal Keratinocytes | T0459

abm | Immortalized Mouse Wildtype M4^+/+ Epidermal Keratinocytes | T0459

+/+ Epidermal Keratinocyte Cells were derived from primary keratinocytes of newborn CD-1 mice. The primary keratinocytes were immortalized in subsequent cultures after passaging for an extended period. The immortalized cells display similar phenotype to the primary keratinocytes including the cobblestone-shape morphology. The Immortalized Mouse Wildtype M4^+/+ Epidermal Keratinocyte Cells are non-tumorigenic. They also differentiate with increased calcium, maintain a relatively stable karyotype no more aberrant than cultured primary keratinocytes, and produce epidermis upon orthotopic grafting onto nude mouse hosts. Upon introduction of v-ras^Ha oncogene, the Immortalized Mouse Wildtype M4^+/+ Epidermal Keratinocyte Cells produce squamous cell carcinoma in orthotopic skin grafts. These cells are useful in studies in epidermal keratinocyte biology and pathology.

Immortalization Method:

Immortalization in subsequent cultures

BioSafety Level:

II

Organism:

CD-1 Mouse

Species:

Mouse

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Keratin 14, Keratin 6

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow XI medium available at abm. , Penicillin/Streptomycin Solution to a final concentration of 1% and 5 ng/ml recombinant human FGF7 (Z100595).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunofluorescence analysis of Keratin markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C