Description
abm | ACΔ3-6 Stable Knockout HEK293 Cell Line | T6291
AC3 and AC6 encoded by the ADCY3 and ADCY6 genes, are the most abundant adenylyl cyclase (AC) isoforms in HEK293 cells. ACΔ3/6 Stable Knockout HEK293 Cell Line was generated by knocking out the AC3 and AC6 genes through stable transfection, selection with puromycin (2 mg/ml), and where then determine to exhibit low cAMP levels.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial-like
Clones:
N/A
Passage Number:
N/A
Population Doner:
24 - 30 hours
Seeding Density:
N/A
Markers:
Puromycin-resistant
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) cAMP detection kit
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C