abm | GALE Stable Knockout HEK293T (Clone 4) Cell Line | T6150

abm | GALE Stable Knockout HEK293T (Clone 4) Cell Line | T6150

The GALE gene encodes UDP-galactose-4 epimerase which operates in the Leloir pathway, the primary pathway for galactose metabolism. This pathway is critical for the generation of UDP-sugars and catalyzes UDP-glucose to UDP-galactose and UDP-N acetylgalactosamine to UDP N-acetylgalactosamine which serve as precursors for O-linked glycosylation and as the major metabolism pathway for galactose. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Embryo

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available from abm

Preservation:

N/A

Quality Control:

1) Confirmed CRISPR-Cas9 presence by GFP examination (Figure 1). 2) Confirmed GALE knockout through western blot analysis and sequencing (Figure S1). Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 3)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C