Description
abm | GALK1 Stable Knockout HEK293T Cell Line | T6153
The Galactokinase 1 (GALK1) is major enzyme in the Leloir pathway, the primary pathway for galactose metabolism. Specifically, this enzyme modifies galactose with a phosphate group to generate galactose 1-phosphate which serves as a precursor for O-linked glycosylation. Deficiencies in GALK1 leads to Galactosemia: a congenital condition that causes galactose to accumulate in blood and tissue.
Biosafety:
II
Organism:
Human
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
18 - 28 hours
Seeding Density:
30,000 - 40,000 cells/cm2
Markers:
N/A
Applications:
For Research Use Only
Doner Gender:
Embryo
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III available from abm
Preservation:
N/A
Quality Control:
1) Confirmed GALK1 knockout through sequencing (Figure S2). Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 4).
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C