abm | GOT2 Knockout Stable HEK293 Cell Line (Clone A3) | T6424

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SKU:
T6424
Availability:
5 to 7 Days Shipment
Size:
10⁶ cells/1.0 ml
Select Currency //4,173.00

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Description

abm | GOT2 Knockout Stable HEK293 Cell Line (Clone A3) | T6424

CRISPR/Cas9 was used to introduce a disruption of the GOT2 gene in HEK293 cells. The resulting CRISPR/Cas9 GOT2-knockout HEK293 cell line is compound heterozygous for 205dup/202_203insA.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

30,000 - 50,000 cells/cm2

Markers:

lacZ-Zeocin, FRT integration site, GOT2 was knocked-out using pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Flow cytometry; 2) Western blot; 3) PCR; 4) Sanger sequencing

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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