Description
abm | GOT2 Knockout Stable HEK293 Cell Line (Clone A7) | T6426
CRISPR/Cas9 was used to introduce a disruption of the GOT2 gene in HEK293 cells. The resulting CRISPR/Cas9 GOT2-knockout HEK293 cell line is homozygous for 205dup.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
30 - 40 hours
Seeding Density:
30,000 - 50,000 cells/cm2
Markers:
lacZ-Zeocin, FRT integration site, GOT2 was knocked-out using pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Preservation:
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) Flow cytometry; 2) Western blot; 3) PCR; 4) Sanger sequencing
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C