Description
abm | ACTB CRISPR Stable HEK293T Cell Line | T6247
β-Actin (ACTB) was integrated into a specific genomic loci using CRISPR/Cas9 in HEK293T cells. The transgene is driven by endogenous promoters to ensure consistent and predictable expression of ACTB. A protein quantification reporter (PQR) was incorporated between the endogenous and RPL13A genes. The PQR allows equimolar production of the endogenous protein, the recombinant protein, and a fluorescent reporter. As a result, expression levels of both the endogenous and recombinant proteins can be continuously monitored using fluorescence.
Biosafety:
II
Organism:
Human
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Polygonal
Clones:
N/A
Passage Number:
N/A
Population Doner:
25 - 35 hours
Seeding Density:
20,000 - 30,000 cells/cm2
Markers:
RFP
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
N/A
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C