abm | AMY3 Stable HEK293 Cell Line | T6257

abm | AMY3 Stable HEK293 Cell Line | T6257

Amylin receptor-3 (AMY3) is an amylin receptor that is suspected to play a role in both Aβ and hAmylin downstream signaling. Amylin receptors belong to class B GPCRs and are heterodimeric complexes, formed by calcitonin receptor (CTR) association with receptor activity modifying proteins (RAMPs). Activation of amylin receptors in mammalian cells has been shown to raise cAMP and presumed to involve GS protein (Gs -guanine nucleotide-binding signal transduction protein) and stimulation of adenylate cyclase. AMY3 Stable HEK293 Cell Line allows for the study of the intracellular signal transduction pathways that are activated by Aβ or hAmylin and to further investigate whether their cytotoxic effects are mediated via this particular isoform of the amylin receptor.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 36 hours

Seeding Density:

80,000 cells/cm²

Markers:

GFP expression, CTR (Calcitonin receptor), RAMP3 (receptor activity modifying protein 3)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Immunohistochemistry; 2) RAMP gene expression; 3) Quantitative cAMP measurements; 4) Western blot; 5) Ca₂ imaging; 6) MTT cell death assay

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C