abm | c-Kit Y568F-Y570F Stable Ba-F3 Cell Line | T3045

abm | c-Kit Y568F-Y570F Stable Ba-F3 Cell Line | T3045

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. Y570 residue, alongside with the Y568 residue, is involved in recruiting signaling molecules, and is part of the autoinhibition process of enzyme activity. c-KitY658/Y570F-expressing Ba/F3 cells (with GNNK- isoform) has a Y-to-F mutation at position 568 and 570. The substitution at both binding sites for signal molecules leads to the loss of Src kinase activation, but has c-kit kinase activity with SCF stimulation. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Together with the wild-type c-Kit stable Ba/F3 cells (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying mutant Y570F human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

1) Western blotting for presence of c-Kit; 2) PCR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C