abm | DN-MEK5 Stably Expressing HCT116 Cell Line | T6445

abm | DN-MEK5 Stably Expressing HCT116 Cell Line | T6445

HCT116 cells were transduced with lentiviral particles carrying pWPI-eGFP expression constructs encoding DN-MEK5, followed by fluorescence-activated cell sorting to generate DN-MEK5 Stably Expressing HCT116 Cell Line. Mitogen/extracellular signal-regulated kinase kinase-5, MEK5, has been associated with tumor carcinogenesis, such as aberrant MEK5–ERK5 pathway signaling have been reported in cancer metastasis. DN-MEK5 Stably Expressing HCT116 Cell Line may be a useful tool to study cancer biology and it's role in cancer invasion and metastasis.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Colon

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

GFP, MEK5

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoat^TM T25 Flasks G299) or Applied Cell Extracellular Matrix G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Cell sorted for GFP expression; 2) Immunoblotting perfromed for MEK5 expression.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C